Recombinant Mouse Anti-Pfs25 Antibody (4B7)
CAT#: FAMAB-0226-CN
4B7 binds P. falciparum surface protein Pfs25, a molecule expressed on the surface of ookinetes, and inhibits parasite development completely when fed to Anopheles mosquitoes in a gametocytemic bloodmeal.
Published Data
Tested Data
IB
Figure 2 Immunoblot of reduced and non-reduced algae-produced Pfs25 with monoclonal transmission blocking antibodies.
Reduced and non-reduced a-Pfs25 was resolved by SDS-PAGE, transferred to nitrocellulose, anddetected with anti-Pfs25 4B7 mAb.
Gregory, J. A., Li, F., Tomosada, L. M., Cox, C. J., Topol, A. B., Vinetz, J. M., & Mayfield, S. (2012). Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PloS one, 7(5), e37179.
WB
Figure 3 Western blot analysis of P.falciparum mixed sexual stage lysates with anti-Pfs25-4B7 mAbs.
Reduced and non-reduced sexual stagelysates were resolved by SDS-PAGE and transferred to nitrocellulose. Blots were probed with anti-Pfs25-4B7 mAbs. (R-reduced, N-non-reduced)
Gregory, J. A., Li, F., Tomosada, L. M., Cox, C. J., Topol, A. B., Vinetz, J. M., & Mayfield, S. (2012). Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PloS one, 7(5), e37179.
IF
Figure 4 Indirect immunofluorescence using immune sera from mice immunized with algae-produced Pfs25 on in-vitro cultured P. falciparum gametocytes, gametes, and zygotes.
DNA was stained using DAPI (blue) and antibody binding was visualized using Alexa Fluor 488-conjugated rabbit anti-mouse IgG (green) for anti-Pfs25 4B7 mAbs. Scale bars, 1 μm.
Gregory, J. A., Li, F., Tomosada, L. M., Cox, C. J., Topol, A. B., Vinetz, J. M., & Mayfield, S. (2012). Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PloS one, 7(5), e37179.
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WB
Figure 1 Recombinant Mouse Anti-Pfs25 Antibody (4B7) in WB.
Western blot analysis of FAMAB-0226-CN was performed by loading recombinant Plasmodium falciparum Pfs25 Antigen (His tag) onto a 15% Tris-HCl polyacrylamide gel. Proteins were transferred to an NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with FAMAB-0226-CN and HRP goat anti-mouse IgG was used as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.5 μg)
Lane 2: Reduced antigen (1.0 μg)
Lane 3: Reduced antigen (1.5 μg)
DB
Figure 2 Recombinant Mouse Anti-Pfs25 Antibody (4B7) in DB.
Dot Blot analysis of FAMAB-0226-CN was performed by coating with Recombinant Plasmodium falciparum Pfs25 Antigen (His tag).
Dot 1: 0.5 μg
Dot 2: 1.0 μg
Dot 3: 1.5 μg
ELISA
Figure 3 Recombinant Mouse Anti-Pfs25 Antibody (4B7) in ELISA.
ELISA analysis of FAMAB-0226-CN was performed by coating with recombinant Plasmodium falciparum Pfs25 Antigen (His tag). Then blocked with BSA and incubated with FAMAB-0226-CN. The HRP-conjugated goat anti-mouse IgG as a secondary antibody.
Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- P. falciparum Pfs25
- Species Reactivity
- P. falciparum
- Clone
- 4B7
- Applications
- ELISA, IB, WB, IF
Product Property
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Immunoblot, Western Blot and Immunofluorescence.
Western Blot: Plasmodium falciparum strain NF54 was maintained in vitro incontinuous cultivation. Gametocytes, macrogametes, zygotes, andookinetes were cultured and purified as previously described. For antibodies raised to a-Pfs25, 2 mg of reduced and nonreducedparasite lysate was prepared as described above and resolved on RUNBLUE 12% SDS-PAGE precast gels. Resolved proteins were transferred to nitrocellulose and probed withantisera raised to a-Pfs25 at a 1:100 dilution. Identical blots were prepared and probed with anti-Pfs25 4B7 mAbs or preimmunesera. All membranes were visualized using AP-conjugated goat anti-mouse IgG antibodies.
Immunofluorescence: Fixed, permeabilized gametocytes, macrogametes, and zygoteswere subjected to IFA. Parasites were heat-fixed onto 10-well glassslides. Fixed cells were permeabilized byincubation in PBS containing 3% bovine serum albumin and 0.1% Triton X-100 at room temperature for 15 minutes followed by blocking for 2 hrs at room temperature in 3% BSA in PBS-T. The preparations were incubated with a-Pfs25 antisera (1:100 dilution) or 4b7 mAbs overnight at 4uC followed by AlexaFluor 488 goat anti-mouse IgG (1:500 dilution)at room temperature for 1 hr. Parasite nuclei were stained with 2mg/mL 49-6-Diamidino-2-phenylindole (DAPI) at room temperature for 5 min. Slides were mountedwith Slow FadeHanti-fade kit and images were acquired using an Applied Precision Spectris microscope and deconvolved using Softworx software. Images were adjusted for contrast in Softworx and exported as tiffs and assembled with Adobe Photoshop.
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(Creative Biolabs Cat# FAMAB-0226-CN, RRID: AB_3111320)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 4B7"
- CAT
- Product Name
See other products for "Pfs25"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0226-CN-F(E) | Recombinant Mouse Anti-Pfs25 Antibody Fab Fragment (4B7) | ELISA, IB | Mouse Fab |
| PABJ-0060-F(E) | Human Anti-Pfs25 Recombinant Antibody (clone 2544); Fab fragment | ELISA, Inhib | Human Fab |
| PABJ-0061-F(E) | Human Anti-Pfs25 Recombinant Antibody (clone 2587); Fab fragment | ELISA | Human Fab |
| PABJ-0062-F(E) | Human Anti-Pfs25 Recombinant Antibody (clone 2530); Fab fragment | ELISA, Block | Human Fab |
| PABJ-0063-F(E) | Human Anti-Pfs25 Recombinant Antibody (clone 2586); Fab fragment | ELISA, Block | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-408 | Recombinant Human Anti-Pfs25 Antibody (1276) | ELISA, WB, BL | Human IgG1 |
| PABJ-0060 | Human Anti-Pfs25 Recombinant Antibody (clone 2544) | ELISA, Inhib | Human IgG |
| PABJ-0061 | Human Anti-Pfs25 Recombinant Antibody (clone 2587) | ELISA | Human IgG |
| PABJ-0062 | Human Anti-Pfs25 Recombinant Antibody (clone 2530) | ELISA, Block | Human IgG |
| PABJ-0063 | Human Anti-Pfs25 Recombinant Antibody (clone 2586) | ELISA, Block | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-408 | Recombinant Human Anti-Pfs25 Antibody scFv Fragment (1276) | ELISA, WB, BL | Human scFv |
| PSBC-409 | Recombinant Human Anti-Pfs25 Antibody scFv Fragment (1269) | ELISA, WB, BL | Human scFv |
| PSBC-410 | Recombinant Human Anti-Pfs25 Antibody scFv Fragment (1260) | ELISA, WB, BL | Human scFv |
| PSBC-411 | Recombinant Human Anti-Pfs25 Antibody scFv Fragment (1245) | ELISA, WB, BL | Human scFv |
| PSBC-412 | Recombinant Human Anti-Pfs25 Antibody scFv Fragment (1262) | ELISA, WB, BL | Human scFv |
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